HomePublicationsDetection and quantification of nanoparticle-induced intracellular ROS in live cells by laser scanning confocal microscopy

Detection and quantification of nanoparticle-induced intracellular ROS in live cells by laser scanning confocal microscopy

  • 8 September 2022
  • Posted by: nemcatgroup
  • Category: Publications
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Graphical abstract

Highlights

  • ROS elevation in HT-1080 by Ce-based nanoparticle treatment.

  • H2DCFDA-mediated detection of ROS by laser scanning confocal microscopy.

  • Writing of custom-built MATLAB software for image analysis of ROS fluorescence intensities.

  • Processing of images for quantification of fluorescence intensities.
  • Statistical analysis by ANOVA to confirm statistical significance.
 

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